January 15, 2012
what would be a good method for identifying the bacteria, which causes leprosy or tuberculosis?
Category: Tuberculosis
what would be a good method for identifying the bacteria, which causes leprosy or tuberculosis?
2 Responses to “what would be a good method for identifying the bacteria, which causes leprosy or tuberculosis?”
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January 15th, 2012 at 12:16 pm
DNA testing of samples.
Can’t grow M lerprae, and M. tuberculosis takes a very, very long time to culture.
January 15th, 2012 at 12:30 pm
ACID FAST STAINING
it is similar in concept to gram staining, but it seperates bacteria into two colors based on whether they’re in the genus Mycobacterium (leprocy/Tuberculosis) versus all other genera.
step 1: apply basic carbolfuchsin dye which is the primary dye, it will stain all cells red but binds stronger to cell wall rich in a certain wax
step 2: wash slide with acid alcohol to decolorize bacteria lacking waxy material
step 3: apply basic stain methylene blue to color all unstained cells blue, this is a counterstain
when all is said and done, the acid fast cells (Mycobacterium) are red, all others are blue
hope that helps!